Upregulation of HLA Expression in Primary Uveal Melanoma by Infiltrating Leukocytes.

ABSTRACT:

Introduction

Uveal melanoma (UM) with an inflammatory phenotype, characterized by infiltrating leukocytes and increased human leukocyte antigen (HLA) expression, carry an increased risk of death due to metastases. These tumors should be ideal for T-cell based therapies, yet it is not clear why prognostically-infaust tumors have a high HLA expression. We set out to determine whether the level of HLA molecules in UM is associated with other genetic factors, HLA transcriptional regulators, or microenvironmental factors.

Methods

28 enucleated UM were used to study HLA class I and II expression, and several regulators of HLA by immunohistochemistry, PCR microarray, qPCR and chromosome SNP-array. Fresh tumor samples of eight primary UM and four metastases were compared to their co

SUBMITTER: van Essen TH 

PROVIDER: S-EPMC5072555 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature