Dynamic Nucleosome Movement Provides Structural Information of Topological Chromatin Domains in Living Human Cells.

ABSTRACT: The mammalian genome is organized into submegabase-sized chromatin domains (CDs) including topologically associating domains, which have been identified using chromosome conformation capture-based methods. Single-nucleosome imaging in living mammalian cells has revealed subdiffusively dynamic nucleosome movement. It is unclear how single nucleosomes within CDs fluctuate and how the CD structure reflects the nucleosome movement. Here, we present a polymer model wherein CDs are characterized by fractal dimensions and the nucleosome fibers fluctuate in a viscoelastic medium with memory. We analytically show that the mean-squared displacement (MSD) of nucleosome fluctuations within CDs is subdiffusive. The diffusion coefficient and the subdiffusive exponent depend on the structural information

SUBMITTER: Shinkai S 

PROVIDER: S-EPMC5072619 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature