Dictyostelium discoideum as a Novel Host System to Study the Interaction between Phagocytes and Yeasts.

ABSTRACT: The social amoeba Dictyostelium discoideum is a well-established model organism to study the interaction between bacteria and phagocytes. In contrast, research using D. discoideum as a host model for fungi is rare. We describe a comprehensive study, which uses D. discoideum as a host model system to investigate the interaction with apathogenic (Saccharomyces cerevisiae) and pathogenic (Candida sp.) yeast. We show that Dictyostelium can be co-cultivated with yeasts on solid media, offering a convenient test to study the interaction between fungi and phagocytes. We demonstrate that a number of D. discoideum mutants increase (atg1^-, kil1^-, kil2^-) or decrease (atg6^-) the abi

SUBMITTER: Koller B 

PROVIDER: S-EPMC5073093 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature