Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides.

ABSTRACT: Rhodotorula (Rhodosporidium) toruloides is an oleaginous yeast with great biotechnological potential, capable of accumulating lipid up to 70% of its dry biomass, and of carotenoid biosynthesis. However, few molecular genetic tools are available for manipulation of this basidiomycete yeast and its high genomic GC content can make routine cloning difficult. We have developed plasmid vectors for transformation of R. toruloides which include elements for Saccharomyces cerevisiae in-yeast assembly; this method is robust to the assembly of GC-rich DNA and of large plasmids. Using such vectors we screened for controllable promoters, and identified inducible promoters from the genes NAR1, ICL1, CTR3, and MET16. These four promoters have independent

SUBMITTER: Johns AM 

PROVIDER: S-EPMC5073140 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature