Targeting erythrocyte carbonic anhydrase and ¹⁸O-isotope of breath CO₂ for sorting out type 1 and type 2 diabetes.

ABSTRACT: The inability to envisage the acute onset and progression of type 1 diabetes (T1D) has been a major clinical stumbling block and an important area of biomedical research over the last few decades. Therefore there is a pressing need to develop a new and an effective strategy for early detection of T1D and to precisely distinguish T1D from type 2 diabetes (T2D). Here we describe the precise role of the enzymatic activity of carbonic anhydrase (CA) in erythrocytes in the pathogenesis of T1D and T2D. We show that CA activities are markedly altered during metabolism of T1D and T2D and this facilitates to the oxygen-18 (¹⁸O) isotopic fractionations of breath CO₂. In our observations, T1D exhibited considerable depletions of ¹⁸O-isotopes of CO_2, whereas

SUBMITTER: Ghosh C 

PROVIDER: S-EPMC5073312 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature