Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems.

ABSTRACT: Alpha subunits of heterotrimeric G proteins (Gα) are involved in a variety of cellular functions. Here we report an optogenetic strategy to spatially and temporally manipulate Gα in living cells. More specifically, we applied the blue light-induced dimerization system, known as the Magnet system, and an alternative red light-induced dimerization system consisting of Arabidopsis thaliana phytochrome B (PhyB) and phytochrome-interacting factor 6 (PIF6) to optically control the activation of two different classes of Gα (Gα_q and Gα_s). By utilizing this strategy, we demonstrate successful regulation of Ca²⁺ and cAMP using light in mammalian cells. The present strategy is generally applicable to different kinds of Gα and could contribute to expanding possibilitie

SUBMITTER: Yu G 

PROVIDER: S-EPMC5073340 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature