Kinesin-5 Contributes to Spindle-length Scaling in the Evolution of Cancer toward Metastasis.

ABSTRACT: During natural evolution, the spindles often scale with cell sizes to orchestrate accurate chromosome segregation. Whether in cancer evolution, when the constraints on genome integrity are relaxed, cancer cells may evolve the spindle to confer other advantages has not been investigated. Using invasion as a selective pressure in vitro, we found that a highly metastatic cancer clone displays a lengthened metaphase spindle, with faster spindle elongation that correlates with transiently elevated speed of cell migration. We found that kinesin-5 is upregulated in this malignant clone, and weak inhibition of kinesin-5 activity could revert the spindle to a smaller aspect ratio, decrease the speed of spindle pole separation, and suppress post-mitotic cell migration. A correlation was found betwee

SUBMITTER: Yang CF 

PROVIDER: S-EPMC5073351 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature