CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell-like Populations and Diverse Mononuclear Phagocytes.

ABSTRACT: CD4⁺ T cells are at the nexus of the innate and adaptive arms of the immune system. However, little is known about the evolutionary history of CD4⁺ T cells, and it is unclear whether their differentiation into specialized subsets is conserved in early vertebrates. In this study, we have created transgenic zebrafish with vibrantly labeled CD4⁺ cells allowing us to scrutinize the development and specialization of teleost CD4⁺ leukocytes in vivo. We provide further evidence that CD4⁺ macrophages have an ancient origin and had already emerged in bony fish. We demonstrate the utility of this zebrafish resource for interrogating the complex behavior of immune cells at cellular resolution by the imaging of intimate contacts between teleost CD

SUBMITTER: Dee CT 

PROVIDER: S-EPMC5073357 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature