Multilocus sequence typing of Trichomonas vaginalis clinical samples from Amsterdam, the Netherlands.

ABSTRACT:

Objectives

In this cross-sectional epidemiological study we aimed to identify molecular profiles for Trichomonas vaginalis and to determine how these molecular profiles were related to patient demographic and clinical characteristics.

Setting

Molecular typing methods previously identified two genetically distinct subpopulations for T. vaginalis; however, few molecular epidemiological studies have been performed. We now increased the sensitivity of a previously described multilocus sequence typing (MLST) tool for T. vaginalis by using nested PCR. This enabled the typing of direct patient samples.

Participants

From January to December 2014, we collected all T. vaginalis positive samples as detected by routine laboratory testing. Samples from patients either came from ge

SUBMITTER: van der Veer C 

PROVIDER: S-EPMC5073662 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature