A comparative study of ChIP-seq sequencing library preparation methods.

ABSTRACT:

Background

ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated DNA must undergo "library preparation" to enable subsequent high-throughput sequencing. To facilitate the analysis of biopsy samples and rare cell populations, there has been a recent proliferation of methods allowing sequencing library preparation from low-input DNA amounts. However, little information exists on the relative merits, performance, comparability and biases inherent to these procedures. Notably, recently developed single-cell ChIP procedures employing microfluidics must also employ library preparation reagents to allow downstream sequencing.

Results

In this study, seven methods designed for low-input DNA/ChIP-seq s

SUBMITTER: Sundaram AY 

PROVIDER: S-EPMC5073829 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature