Viral recombination blurs taxonomic lines: examination of single-stranded DNA viruses in a wastewater treatment plant.

ABSTRACT: Understanding the structure and dynamics of microbial communities, especially those of economic concern, is of paramount importance to maintaining healthy and efficient microbial communities at agricultural sites and large industrial cultures, including bioprocessors. Wastewater treatment plants are large bioprocessors which receive water from multiple sources, becoming reservoirs for the collection of many viral families that infect a broad range of hosts. To examine this complex collection of viruses, full-length genomes of circular ssDNA viruses were isolated from a wastewater treatment facility using a combination of sucrose-gradient size selection and rolling-circle amplification and sequenced on an Illumina MiSeq. Single-stranded DNA viruses are among the least understood groups of m

SUBMITTER: Pearson VM 

PROVIDER: S-EPMC5075696 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature