Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors.

ABSTRACT: The stringent response is a central adaptation mechanism that allows bacteria to adjust their growth and metabolism according to environmental conditions. The functionality of the stringent response is crucial for bacterial virulence, survival during host invasion as well as antibiotic resistance and tolerance. Therefore, specific inhibitors of the stringent response hold great promise as molecular tools for disarming and pacifying bacterial pathogens. By taking advantage of the valine amino acid auxotrophy of the Bacillus subtilis stringent response-deficient strain, we have set up a High Throughput Screening assay for the identification of stringent response inhibitors. By screening 17,500 compounds, we have identified a novel class of antibacterials based on the 4-(6-(phenoxy)alkyl)-3,5

SUBMITTER: Andresen L 

PROVIDER: S-EPMC5075769 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature