A high-throughput microfluidic approach for 1000-fold leukocyte reduction of platelet-rich plasma.

ABSTRACT: Leukocyte reduction of donated blood products substantially reduces the risk of a number of transfusion-related complications. Current 'leukoreduction' filters operate by trapping leukocytes within specialized filtration material, while allowing desired blood components to pass through. However, the continuous release of inflammatory cytokines from the retained leukocytes, as well as the potential for platelet activation and clogging, are significant drawbacks of conventional 'dead end' filtration. To address these limitations, here we demonstrate our newly-developed 'controlled incremental filtration' (CIF) approach to perform high-throughput microfluidic removal of leukocytes from platelet-rich plasma (PRP) in a continuous flow regime. Leukocytes are separated from platelets within the P

SUBMITTER: Xia H 

PROVIDER: S-EPMC5075940 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature