α-Aminoadipic acid protects against retinal disruption through attenuating Muller cell gliosis in a rat model of acute ocular hypertension.

ABSTRACT:

Objective

Ocular hypertension is an important risk factor for glaucoma. The purpose of this study was to investigate the gliotoxic effects of α-aminoadipic acid (AAA) in a rat model of AOH and its underlying mechanisms.

Materials and methods

In the rat model of acute ocular hypertension (AOH), intraocular pressure was increased to 110 mmHg for 60 minutes. Animals were divided into four groups: sham operation (Ctrl), AOH, AOH + phosphate-buffered saline (PBS), and AOH + AAA. Cell apoptosis in the ganglion cell layer was detected with the terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling (TUNEL) assay, and retinal ganglion cells (RGCs) immunostained with Thy-1 were counted. Müller cell activation was detected using immunostaining w

SUBMITTER: Wang X 

PROVIDER: S-EPMC5076852 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature