Chloroplast sequence of treegourd (Crescentia cujete, Bignoniaceae) to study phylogeography and domestication.

ABSTRACT:

Premise of the study

Crescentia cujete (Bignoniaceae) fruit rinds are traditionally used for storage vessels and handicrafts. We assembled its chloroplast genome and identified single-nucleotide polymorphisms (SNPs).

Methods and results

Using a genome skimming approach, the whole chloroplast of C. cujete was assembled using 3,106,928 sequence reads of 150 bp. The chloroplast is 154,662 bp in length, structurally divided into a large single copy region (84,788 bp), a small single copy region (18,299 bp), and two inverted repeat regions (51,575 bp) with 88 genes annotated. By resequencing the whole chloroplast, we identified 66 SNPs in C. cujete (N = 30) and 68 SNPs in C. amazonica (N = 6). Nucleotide diversity was estimated at 1.1 × 1

SUBMITTER: Moreira PA 

PROVIDER: S-EPMC5077280 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature