The mutational spectrum of FOXA2 in endometrioid endometrial cancer points to a tumor suppressor role.

ABSTRACT:

Background

Forkhead box protein A2 (FOXA2) plays an important in development, cellular metabolism and tumorigenesis. The Cancer Genome Atlas (TCGA) identified a modest frequency of FOXA2 mutations in endometrioid endometrial cancers (EEC). The current study sought to determine the relationship between FOXA2 mutation and clinicopathologic features in EEC and FOXA2 expression.

Methods

Polymerase chain reaction (PCR) amplification and sequencing were used to identify mutations in 542 EEC. Western blot, quantitative reverse transcriptase PCR (qRT-PCR) and immunohistochemistry (IHC) were used to assess expression. Methylation analysis was performed using combined bisulfite restriction analysis (COBRA) and sequencing. Chi-squared, Fisher's exact, Student's t- and log-rank tests we

SUBMITTER: Smith B 

PROVIDER: S-EPMC5077662 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature