Nascent Proteome Remodeling following Homeostatic Scaling at Hippocampal Synapses.

ABSTRACT: Homeostatic scaling adjusts the strength of synaptic connections up or down in response to large changes in input. To identify the landscape of proteomic changes that contribute to opposing forms of homeostatic plasticity, we examined the plasticity-induced changes in the newly synthesized proteome. Cultured rat hippocampal neurons underwent homeostatic up-scaling or down-scaling. We used BONCAT (bio-orthogonal non-canonical amino acid tagging) to metabolically label, capture, and identify newly synthesized proteins, detecting and analyzing 5,940 newly synthesized proteins using mass spectrometry and label-free quantitation. Neither up- nor down-scaling produced changes in the number of different proteins translated. Rather, up- and down-scaling elicited opposing translational regulation o

SUBMITTER: Schanzenbacher CT 

PROVIDER: S-EPMC5078608 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature