Novel Syntrophic Populations Dominate an Ammonia-Tolerant Methanogenic Microbiome.

ABSTRACT: Biogas reactors operating with protein-rich substrates have high methane potential and industrial value; however, they are highly susceptible to process failure because of the accumulation of ammonia. High ammonia levels cause a decline in acetate-utilizing methanogens and instead promote the conversion of acetate via a two-step mechanism involving syntrophic acetate oxidation (SAO) to H₂ and CO₂, followed by hydrogenotrophic methanogenesis. Despite the key role of syntrophic acetate-oxidizing bacteria (SAOB), only a few culturable representatives have been characterized. Here we show that the microbiome of a commercial, ammonia-tolerant biogas reactor harbors a deeply branched, uncultured phylotype (unFirm_1) accounting for approximately 5% of the 16S rRNA gene inven

SUBMITTER: Frank JA 

PROVIDER: S-EPMC5080403 | biostudies-literature | 2016 Sep-Oct

REPOSITORIES: biostudies-literature