Transcriptomic analysis of the ion channelome of human platelets and megakaryocytic cell lines.

ABSTRACT: Ion channels have crucial roles in all cell types and represent important therapeutic targets. Approximately 20 ion channels have been reported in human platelets; however, no systematic study has been undertaken to define the platelet channelome. These membrane proteins need only be expressed at low copy number to influence function and may not be detected using proteomic or transcriptomic microarray approaches. In our recent work, quantitative real-time PCR (qPCR) provided key evidence that Kv1.3 is responsible for the voltage-dependent K+ conductance of platelets and megakaryocytes. The present study has expanded this approach to assess relative expression of 402 ion channels and channel regulatory genes in human platelets and three megakaryoblastic/erythroleukaemic cell lines. mRNA lev

SUBMITTER: Wright JR 

PROVIDER: S-EPMC5080539 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature