Valproate inhibits MAP kinase signalling and cell cycle progression in S. cerevisiae.

ABSTRACT: The mechanism of action of valproate (VPA), a widely prescribed short chain fatty acid with anticonvulsant and anticancer properties, remains poorly understood. Here, the yeast Saccharomyces cerevisiae was used as model to investigate the biological consequences of VPA exposure. We found that low pH strongly potentiates VPA-induced growth inhibition. Transcriptional profiling revealed that under these conditions, VPA modulates the expression of genes involved in diverse cellular processes including protein folding, cell wall organisation, sexual reproduction, and cell cycle progression. We further investigated the impact of VPA on selected processes and found that this drug: i) activates markers of the unfolded protein stress response such as Hac1 mRNA splicing; ii) modulates the cell wall

SUBMITTER: Desfosses-Baron K 

PROVIDER: S-EPMC5080547 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature