Cyclin A1 expression and paclitaxel resistance in human ovarian cancer cells.

ABSTRACT:

Background

The development of intrinsic and acquired resistance to antineoplastic agents is a major obstacle to successful chemotherapy in ovarian cancers. Identification and characterisation of chemoresponse-associated biomarkers are of paramount importance for novel therapeutic development.

Methods

Global RNA expression profiles were obtained by high-throughput microarray analysis. Cell cycle, proliferation rate, and paclitaxel sensitivity of ovarian cancer cells harbouring cyclin A1-inducible expression construct were compared with and without tetracycline induction, as well as when the cyclin A1 expression was suppressed by short inhibiting RNA (siRNA). Cellular senescence was evaluated by β-galactosidase activity staining.

Results

Global RNA expression profiling

SUBMITTER: Huang KC 

PROVIDER: S-EPMC5080661 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature