Hypoxia increases genome-wide bivalent epigenetic marking by specific gain of H3K27me3.

ABSTRACT:

Background

Trimethylation at histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3) controls gene activity during development and differentiation. Whether H3K4me3 and H3K27me3 changes dynamically in response to altered microenvironmental conditions, including low-oxygen conditions commonly present in solid tumors, is relatively unknown. Demethylation of H3K4me3 and H3K27me3 is mediated by oxygen and 2-oxoglutarate dioxygenases enzymes, suggesting that oxygen deprivation (hypoxia) may influence histone trimethylation. Using the MCF7 breast epithelial adenocarcinoma cell model, we have determined the relationship between epigenomic and transcriptomic reprogramming as a function of fluctuating oxygen tension.

Results

We find that in MCF7, H3K4me3 and H3K27me3 marks rapidly incr

SUBMITTER: Prickaerts P 

PROVIDER: S-EPMC5080723 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature