Real time PCR for the rapid identification and drug susceptibility of Mycobacteria present in Bronchial washings.

ABSTRACT:

Background

Mycobacteria have a spectrum of virulence and different susceptibilities to antibiotics. Distinguishing mycobacterial species is vital as patients with non-tuberculous mycobacterial (NTM) infections present clinical features that are similar to those of patients with tuberculosis. Thus, rapid differentiation of Mycobacterium tuberculosis complex from NTM is critical to administer appropriate treatment. Hence the aim of the study was to rapid identification of mycobacterial species present in bronchial washings using multiplex real time Polymerase Chain Reaction (PCR) and to determine the drug susceptibility in identified mycobacterial species.

Methods

Sputum smear negative bronchoscopy specimens (n = 150) were collected for a period of one year, from patients atte

SUBMITTER: Keerthirathne TP 

PROVIDER: S-EPMC5080729 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature