Chromatin Kinases Act on Transcription Factors and Histone Tails in Regulation of Inducible Transcription.

ABSTRACT: The inflammatory response requires coordinated activation of both transcription factors and chromatin to induce transcription for defense against pathogens and environmental insults. We sought to elucidate the connections between inflammatory signaling pathways and chromatin through genomic footprinting of kinase activity and unbiased identification of prominent histone phosphorylation events. We identified H3 serine 28 phosphorylation (H3S28ph) as the principal stimulation-dependent histone modification and observed its enrichment at induced genes in mouse macrophages stimulated with bacterial lipopolysaccharide. Using pharmacological and genetic approaches, we identified mitogen- and stress-activated protein kinases (MSKs) as primary mediators of H3S28ph in macrophages. Cell-free transcr

SUBMITTER: Josefowicz SZ 

PROVIDER: S-EPMC5081221 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature