A Comparative Proteome Analysis of Escherichia coli ΔrelA Mutant Cells.

ABSTRACT: The bacterial RelA-dependent stringent response exerts a strong influence over various processes. In this work, the impact of the relA gene mutation in Escherichia coli cells was evaluated by a quantitative proteomics analysis, employing stable-isotope labeling and high-resolution mass spectrometry. Chemostat cultures of E. coli W3110 and ΔrelA mutant strains were performed at two dilution rates (0.1 and 0.2 h^-1) to assess the influence of the relA gene mutation in steady-state protein levels. A total of 121 proteins showed significant alterations in their abundance when comparing the proteome of mutant to wild-type cells. The relA gene mutation induced changes on key cellular processes, including the amino acids and nucleotide biosynthe

SUBMITTER: Carneiro S 

PROVIDER: S-EPMC5081369 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature