Suppressing hyperinsulinemia prevents obesity but causes rapid onset of diabetes in leptin-deficient Lep^ob/ob mice.

ABSTRACT:

Objective

Hyperinsulinemia is commonly associated with obesity. Mice deficient in the adipose-derived hormone leptin (Lep^ob/ob) develop hyperinsulinemia prior to onset of obesity and glucose intolerance. Whether the excess of circulating insulin is a major contributor to obesity and impaired glucose homeostasis in Lep^ob/ob mice is unclear. It has been reported previously that diet-induced obesity in mice can be prevented by reducing insulin gene dosage. In the present study, we examined the effects of genetic insulin reduction in Lep^ob/ob mice on circulating insulin, body composition, and glucose homeostasis.

Methods

Leptin expressing (Lep^wt/wt) mice lacking 3 insulin alleles were crossed with Lep

SUBMITTER: D'souza AM 

PROVIDER: S-EPMC5081422 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature