Transcriptome-Wide Analysis Reveals the Role of PPARγ Controlling the Lipid Metabolism in Goat Mammary Epithelial Cells.

ABSTRACT: To explore the large-scale effect of peroxisome proliferator-activated receptor γ (PPARG) in goat mammary epithelial cells (GMEC), an oligonucleotide microarray platform was used for transcriptome profiling in cells overexpressing PPARG and incubated with or without rosiglitazone (ROSI, a PPARγ agonist). A total of 1143 differentially expressed genes (DEG) due to treatment were detected. The Dynamic Impact Approach (DIA) analysis uncovered the most impacted and induced pathways "fatty acid elongation in mitochondria," "glycosaminoglycan biosynthesis-keratan sulfate," and "pentose phosphate pathway." The data highlights the central role of PPARG in milk fatty acid metabolism via controlling fatty acid elongation, biosynthesis of unsaturated fatty acid, lip

SUBMITTER: Shi H 

PROVIDER: S-EPMC5081438 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature