A gene-centered C. elegans protein-DNA interaction network provides a framework for functional predictions.

ABSTRACT: Transcription factors (TFs) play a central role in controlling spatiotemporal gene expression and the response to environmental cues. A comprehensive understanding of gene regulation requires integrating physical protein-DNA interactions (PDIs) with TF regulatory activity, expression patterns, and phenotypic data. Although great progress has been made in mapping PDIs using chromatin immunoprecipitation, these studies have only characterized ~10% of TFs in any metazoan species. The nematode C. elegans has been widely used to study gene regulation due to its compact genome with short regulatory sequences. Here, we delineated the largest gene-centered metazoan PDI network to date by examining interactions between 90% of C. elegans TFs and 15% of gene promoters. We used this network as a backb

SUBMITTER: Fuxman Bass JI 

PROVIDER: S-EPMC5081483 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature