Revealing the diversity of extracellular vesicles using high-dimensional flow cytometry analyses.

ABSTRACT: Extracellular vesicles (EV) are small membrane vesicles produced by cells upon activation and apoptosis. EVs are heterogeneous according to their origin, mode of release, membrane composition, organelle and biochemical content, and other factors. Whereas it is apparent that EVs are implicated in intercellular communication, they can also be used as biomarkers. Continuous improvements in pre-analytical parameters and flow cytometry permit more efficient assessment of EVs; however, methods to more objectively distinguish EVs from cells and background, and to interpret multiple single-EV parameters are lacking. We used spanning-tree progression analysis of density-normalized events (SPADE) as a computational approach for the organization of EV subpopulations released by platelets and erythroc

SUBMITTER: Marcoux G 

PROVIDER: S-EPMC5081512 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature