Label-free imaging of the native, living cellular nanoarchitecture using partial-wave spectroscopic microscopy.

ABSTRACT: The organization of chromatin is a regulator of molecular processes including transcription, replication, and DNA repair. The structures within chromatin that regulate these processes span from the nucleosomal (10-nm) to the chromosomal (>200-nm) levels, with little known about the dynamics of chromatin structure between these scales due to a lack of quantitative imaging technique in live cells. Previous work using partial-wave spectroscopic (PWS) microscopy, a quantitative imaging technique with sensitivity to macromolecular organization between 20 and 200 nm, has shown that transformation of chromatin at these length scales is a fundamental event during carcinogenesis. As the dynamics of chromatin likely play a critical regulatory role in cellular function, it is critical to develop live

SUBMITTER: Almassalha LM 

PROVIDER: S-EPMC5081614 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature