Differential effects of altered patterns of movement and strain on joint cell behaviour and skeletal morphogenesis.

ABSTRACT:

Objective

There is increasing evidence that joint shape is a potent predictor of osteoarthritis (OA) risk; yet the cellular events underpinning joint morphogenesis remain unclear. We sought to develop a genetically tractable animal model to study the events controlling joint morphogenesis.

Design

Zebrafish larvae were subjected to periods of flaccid paralysis, rigid paralysis or hyperactivity. Immunohistochemistry and transgenic reporters were used to monitor changes to muscle and cartilage. Finite Element Models were generated to investigate the mechanical conditions of rigid paralysis. Principal component analysis was used to test variations in skeletal morphology and metrics for shape, orientation and size were applied to describe cell behaviour.

Results

We show th

SUBMITTER: Brunt LH 

PROVIDER: S-EPMC5081689 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature