Regulatory mechanisms of TGF-β1-induced fibrogenesis of human alveolar epithelial cells.

ABSTRACT: Pulmonary fibrosis is characterized by an extensive activation of fibrogenic cells and deposition of extracellular matrix (ECM). Transforming growth factor (TGF)-β1 plays a pivotal role in the pathogenesis of pulmonary fibrosis, probably through the epithelial- to-mesenchymal transition (EMT) and ECM production. The present study investigates potential mechanism by which TGF-β1 induces EMT and ECM production in the fibrogenesis of human lung epithelial cells during pulmonary fibrosis. The expression of EMT phenotype and other proteins relevant to fibrogenesis were measured and the cell bio-behaviours were assessed using Cell-IQ Alive Image Monitoring System. We found that TGF-β1-induced EMT was accompanied with increased collagen I deposition, which may be involved in the regulation of con

SUBMITTER: Shi L 

PROVIDER: S-EPMC5082411 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature