The Impact of Environmental and Endogenous Damage on Somatic Mutation Load in Human Skin Fibroblasts.

ABSTRACT: Accumulation of somatic changes, due to environmental and endogenous lesions, in the human genome is associated with aging and cancer. Understanding the impacts of these processes on mutagenesis is fundamental to understanding the etiology, and improving the prognosis and prevention of cancers and other genetic diseases. Previous methods relying on either the generation of induced pluripotent stem cells, or sequencing of single-cell genomes were inherently error-prone and did not allow independent validation of the mutations. In the current study we eliminated these potential sources of error by high coverage genome sequencing of single-cell derived clonal fibroblast lineages, obtained after minimal propagation in culture, prepared from skin biopsies of two healthy adult humans. We report

SUBMITTER: Saini N 

PROVIDER: S-EPMC5082821 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature