Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy.

ABSTRACT: Mutations in genes encoding components of the sarcolemmal dystrophin-glycoprotein complex (DGC) are responsible for a large number of muscular dystrophies. As such, molecular dissection of the DGC is expected to both reveal pathological mechanisms, and provides a biological framework for validating new DGC components. Establishment of the molecular composition of plasma-membrane protein complexes has been hampered by a lack of suitable biochemical approaches. Here we present an analytical workflow based upon the principles of protein correlation profiling that has enabled us to model the molecular composition of the DGC in mouse skeletal muscle. We also report our analysis of protein complexes in mice harboring mutations in DGC components. Bioinformatic analyses suggested that cell-adhesio

SUBMITTER: Turk R 

PROVIDER: S-EPMC5083101 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature