The development of modified human Hsp70 (HSPA1A) and its production in the milk of transgenic mice.

ABSTRACT: The production of major human heat shock protein Hsp70 (HSPA1A) in a eukaryotic expression system is needed for testing and possible medical applications. In this study, transgenic mice were produced containing wild-type human Hsp70 allele in the vector providing expression in the milk. The results indicated that human Hsp70 was readily expressed in the transgenic animals but did not apparently preserve its intact structure and, hence, it was not possible to purify the protein using conventional isolation techniques. It was suggested that the protein underwent glycosylation in the process of expression, and this quite common modification for proteins expressed in the milk complicated its isolation. To check this possibility, we mutated all presumptive sites of glycosylation and tested the

SUBMITTER: Gurskiy YG 

PROVIDER: S-EPMC5083674 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature