Somatic Mutations Modulate Autoantibodies against Galactose-Deficient IgA1 in IgA Nephropathy.

ABSTRACT: Autoantibodies against galactose-deficient IgA1 drive formation of pathogenic immune complexes in IgA nephropathy. IgG autoantibodies against galactose-deficient IgA1 in patients with IgA nephropathy have a specific amino-acid sequence, Y₁CS₃, in the complementarity-determining region 3 of the heavy chain variable region compared with a Y₁CA₃ sequence in similar isotype-matched IgG from healthy controls. We previously found that the S₃ residue is critical for binding galactose-deficient IgA1. To determine whether this difference is due to a rare germline sequence, we amplified and sequenced the corresponding germline variable region genes from peripheral blood mononuclear cells of seven patients with IgA nephropathy and six healthy con

SUBMITTER: Huang ZQ 

PROVIDER: S-EPMC5084875 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature