Vasopressin Regulates Extracellular Vesicle Uptake by Kidney Collecting Duct Cells.

ABSTRACT: Extracellular vesicles (ECVs) facilitate intercellular communication along the nephron, with the potential to change the function of the recipient cell. However, it is not known whether this is a regulated process analogous to other signaling systems. We investigated the potential hormonal regulation of ECV transfer and report that desmopressin, a vasopressin analogue, stimulated the uptake of fluorescently loaded ECVs into a kidney collecting duct cell line (mCCD_C11) and into primary cells. Exposure of mCCD_C11 cells to ECVs isolated from cells overexpressing microRNA-503 led to downregulated expression of microRNA-503 target genes, but only in the presence of desmopressin. Mechanistically, ECV entry into mCCD_C11 cells required cAMP production, was reduced

SUBMITTER: Oosthuyzen W 

PROVIDER: S-EPMC5084879 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature