The Ebola virus protein VP40 hexamer enhances the clustering of PI(4,5)P₂ lipids in the plasma membrane.

ABSTRACT: The Ebola virus is a lipid-enveloped virus that obtains its lipid coat from the plasma membrane of the host cell it infects during the budding process. The Ebola virus protein VP40 localizes to the inner leaflet of the plasma membrane and forms the viral matrix, which provides the major structure for the Ebola virus particles. VP40 is initially a dimer that rearranges to a hexameric structure that mediates budding. VP40 hexamers and larger filaments have been shown to be stabilized by PI(4,5)P₂ in the plasma membrane inner leaflet. Reduction in the plasma membrane levels of PI(4,5)P₂ significantly reduce formation of VP40 oligomers and virus-like particles. We investigated the lipid-protein interactions in VP40 hexamers at the plasma membrane. We quantified lipid-lipi

SUBMITTER: Gc JB 

PROVIDER: S-EPMC5084917 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature