De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.

ABSTRACT: A major goal in metalloprotein design is to build protein scaffolds from scratch that allow precise control over metal coordination. A particular challenge in this regard is the construction of allosteric systems in which metal coordination equilibria are coupled to other chemical events that take place elsewhere in the protein scaffold. We previously developed a metal-templated self-assembly strategy (MeTIR) to build supramolecular protein complexes with tailorable interfaces from monomeric building blocks. Here, using this strategy, we have incorporated multiple disulfide bonds into the interfaces of a Zn-templated cytochrome cb₅₆₂ assembly in order to create mechanical strain on the quaternary structural level. Structural and biophysical analyses indicate that this strain lea

SUBMITTER: Churchfield LA 

PROVIDER: S-EPMC5085265 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature