Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model.

ABSTRACT:

Background

Herein we describe a small-diameter vascular graft constructed from rolled human amniotic membrane (hAM), with in vitro evaluation and subsequent in vivo assessment of its mechanical and initial biologic viability in the early postimplantation period. This approach for graft construction allows customization of graft dimensions, with wide-ranging potential clinical applicability as a nonautologous, allogeneic, cell-free graft material.

Methods

Acellular hAMs were rolled into layered conduits (3.2-mm diameter) that were bound with fibrin and lyophilized. Constructs were seeded with human smooth muscle cells and cultured under controlled arterial hemodynamic conditions in vitro. Additionally, the acellular hAM conduits were surgically implanted as arterial interposi

SUBMITTER: Amensag S 

PROVIDER: S-EPMC5086323 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature