Microcystin-Bound Protein Patterns in Different Cultures of Microcystis aeruginosa and Field Samples.

ABSTRACT: Micocystin (MC) exists in Microcystis cells in two different forms, free and protein-bound. We examined the dynamic change in extracellular free MCs, intracellular free MCs and protein-bound MCs in both batch cultures and semi-continuous cultures, using high performance liquid chromatography and Western blot. The results showed that the free MC per cell remained constant, while the quantity of protein-bound MCs increased with the growth of Microcystis cells in both kinds of culture. Significant changes in the dominant MC-bound proteins occurred in the late exponential growth phase of batch cultures, while the dominant MC-bound proteins in semi-continuous cultures remained the same. In field samples collected at different months in Lake Taihu, the dominant MC-bound proteins we

SUBMITTER: Wei N 

PROVIDER: S-EPMC5086653 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature