Identifying reproducible cancer-associated highly expressed genes with important functional significances using multiple datasets.

ABSTRACT: Identifying differentially expressed (DE) genes between cancer and normal tissues is of basic importance for studying cancer mechanisms. However, current methods, such as the commonly used Significance Analysis of Microarrays (SAM), are biased to genes with low expression levels. Recently, we proposed an algorithm, named the pairwise difference (PD) algorithm, to identify highly expressed DE genes based on reproducibility evaluation of top-ranked expression differences between paired technical replicates of cells under two experimental conditions. In this study, we extended the application of the algorithm to the identification of DE genes between two types of tissue samples (biological replicates) based on several independent datasets or sub-datasets of a dataset, by constructing multiple

SUBMITTER: Huang H 

PROVIDER: S-EPMC5086981 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature