Bicarbonate-induced redox tuning in Photosystem II for regulation and protection.

ABSTRACT: The midpoint potential (E_m) of [Formula: see text], the one-electron acceptor quinone of Photosystem II (PSII), provides the thermodynamic reference for calibrating PSII bioenergetics. Uncertainty exists in the literature, with two values differing by ∼80 mV. Here, we have resolved this discrepancy by using spectroelectrochemistry on plant PSII-enriched membranes. Removal of bicarbonate (HCO₃^-) shifts the E_m from ∼-145 mV to -70 mV. The higher values reported earlier are attributed to the loss of HCO₃^- during the titrations (pH 6.5, stirred under argon gassing). These findings mean that HCO₃^- binds less strongly when Q_A^-• is present. Light-induced Q_A^-• formati

SUBMITTER: Brinkert K 

PROVIDER: S-EPMC5087022 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature