Chromatin profiling of Drosophila CNS subpopulations identifies active transcriptional enhancers.

ABSTRACT: One of the key issues in studying transcriptional regulation during development is how to employ genome-wide assays that reveals sites of open chromatin and transcription factor binding to efficiently identify biologically relevant genes and enhancers. Analysis of Drosophila CNS midline cell development provides a useful system for studying transcriptional regulation at the genomic level due to a large, well-characterized set of midline-expressed genes and in vivo validated enhancers. In this study, FAIRE-seq on FACS-purified midline cells was performed and the midline FAIRE data were compared with whole-embryo FAIRE data. We find that regions of the genome with a strong midline FAIRE peak and weak whole-embryo FAIRE peak overlap with known midline enhancers and provide a useful predictive

SUBMITTER: Pearson JC 

PROVIDER: S-EPMC5087646 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature