Zebrafish P54 RNA helicases are cytoplasmic granule residents that are required for development and stress resilience.

ABSTRACT: Stress granules are cytoplasmic foci that directly respond to the protein synthesis status of the cell. Various environmental insults, such as oxidative stress or extreme heat, block protein synthesis; consequently, mRNA will stall in translation, and stress granules will immediately form and become enriched with mRNAs. P54 DEAD box RNA helicases are components of RNA granules such as P-bodies and stress granules. We studied the expression, in cytoplasmic foci, of both zebrafish P54 RNA helicases (P54a and P54b) during development and found that they are expressed in cytoplasmic granules under both normal conditions and stress conditions. In zebrafish embryos exposed to heat shock, some proportion of P54a and P54b helicases move to larger granules that exhibit the properties of genuine str

SUBMITTER: Zampedri C 

PROVIDER: S-EPMC5087673 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature