Strategic Positioning and Biased Activity of the Mitochondrial Calcium Uniporter in Cardiac Muscle.

ABSTRACT: Control of myocardial energetics by Ca²⁺ signal propagation to the mitochondrial matrix includes local Ca²⁺ delivery from sarcoplasmic reticulum (SR) ryanodine receptors (RyR2) to the inner mitochondrial membrane (IMM) Ca²⁺ uniporter (mtCU). mtCU activity in cardiac mitochondria is relatively low, whereas the IMM surface is large, due to extensive cristae folding. Hence, stochastically distributed mtCU may not suffice to support local Ca²⁺ transfer. We hypothesized that mtCU concentrated at mitochondria-SR associations would promote the effective Ca²⁺ transfer. mtCU distribution was determined by tracking MCU and EMRE, the proteins essential for channel formation. Both proteins were enriched in the IMM-outer mitochondrial membrane (OMM

SUBMITTER: De La Fuente S 

PROVIDER: S-EPMC5087749 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature