Coassembly of Peptides Derived from β-Sheet Regions of β-Amyloid.

ABSTRACT: In this paper, we investigate the coassembly of peptides derived from the central and C-terminal regions of the β-amyloid peptide (Aβ). In the preceding paper, J. Am. Chem. Soc. 2016, DOI: 10.1021/jacs.6b06000 , we established that peptides containing residues 17-23 (LVFFAED) from the central region of Aβ and residues 30-36 (AIIGLMV) from the C-terminal region of Aβ assemble to form homotetramers consisting of two hydrogen-bonded dimers. Here, we mix these tetramer-forming peptides and determine how they coassemble. Incorporation of a single ¹⁵N isotopic label into each peptide provides a spectroscopic probe with which to elucidate the coassembly of the peptides by ¹H,¹⁵N HSQC. Job's method of continuous variation and nonlinear least-squares fitting reveal

SUBMITTER: Truex NL 

PROVIDER: S-EPMC5089069 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature