Podosome dynamics and location in vascular smooth muscle cells require CLASP-dependent microtubule bending.

ABSTRACT: Extracellular matrix (ECM) remodeling during physiological processes is mediated by invasive protrusions called podosomes. Positioning and dynamics of podosomes define the extent of ECM degradation. Microtubules are known to be involved in podosome regulation, but the role of microtubule (MT) network configuration in podosome dynamics and positioning is not well understood. Here, we show that the arrangement of the microtubule network defines the pattern of podosome formation and relocation in vascular smooth muscle cells (VSMCs). We show that microtubule plus-end targeting facilitates de novo formation of podosomes, in addition to podosome remodeling. Moreover, specialized bent microtubules with plus ends reversed towards the cell center promote relocation of podosomes from the cell edge

SUBMITTER: Zhu X 

PROVIDER: S-EPMC5089163 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature