A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.

ABSTRACT: The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive interplay between these processes. By monitoring dynamic changes in the centrosome-cilium protein interaction landscape during ciliogenesis, we also identify satellite proteins that support cilia formation. Systematic

SUBMITTER: Gupta GD 

PROVIDER: S-EPMC5089374 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature