Dual Detection System for Simultaneous Measurement of Intracellular Fluorescent Markers and Cellular Secretion.

ABSTRACT: Glucose-stimulated insulin secretion from pancreatic β-cells within islets of Langerhans plays a critical role in maintaining glucose homeostasis. Although this process is essential for maintaining euglycemia, the underlying intracellular mechanisms that control it are still unclear. To allow simultaneous correlation between intracellular signal transduction events and extracellular secretion, an analytical system was developed that integrates fluorescence imaging of intracellular probes with high-speed automated insulin immunoassays. As a demonstration of the system, intracellular [Ca²⁺] ([Ca²⁺]_i) was measured by imaging Fura-2 fluorescence simultaneously with insulin secretion from islets exposed to elevated glucose levels. Both [Ca²⁺]_i

SUBMITTER: Yi L 

PROVIDER: S-EPMC5089909 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature